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Related Concept Videos

Complement System01:27

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The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
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Related Experiment Video

Updated: Jan 5, 2026

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion
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Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

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Complement component C1q is produced by isolated articular chondrocytes.

R Lubbers1, R A van Schaarenburg2, J C Kwekkeboom1

  • 1Department of Rheumatology, Leiden University Medical Center, Leiden, the Netherlands.

Osteoarthritis and Cartilage
|October 22, 2019
PubMed
Summary
This summary is machine-generated.

Human articular chondrocytes produce and secrete C1q protein, which binds to these cells and influences collagen expression, potentially impacting osteoarthritis development.

Keywords:
C1qCartilageChondrocytesComplementOsteoarthritis

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Area of Science:

  • Biochemistry
  • Immunology
  • Cell Biology

Background:

  • Inflammation and innate immune responses are implicated in osteoarthritis (OA) development and progression.
  • Chondrocytes, the cells of articular cartilage, produce extracellular matrix molecules, but how inflammatory mediators reach them is unclear.
  • Previous studies suggested local C1q protein production by chondrocytes, but this remained unconfirmed.

Purpose of the Study:

  • To investigate C1q protein production by human articular chondrocytes.
  • To determine if chondrocytes secrete C1q protein.
  • To explore the effects of C1q binding on chondrocytes.

Main Methods:

  • Analysis of C1q protein expression in human articular chondrocytes using Western blot, ELISA, and flow cytometry.
  • Examination of mRNA expression changes in collagen, MMP-1, and complement genes upon stimulation.
  • Assessment of C1 gene expression in mouse articular chondrocytes.

Main Results:

  • Human articular chondrocytes express and secrete C1q protein into the extracellular medium.
  • Pro-inflammatory cytokine stimulation upregulated C1q subunit mRNA but not protein levels.
  • Extracellular C1q dose-dependently bound to chondrocytes, altering collagen type 2 and type 10 expression.

Conclusions:

  • Human articular chondrocytes express and secrete C1q protein.
  • Secreted C1q can bind to chondrocytes, influencing their collagen expression profile.
  • These findings suggest a role for chondrocyte-derived C1q in cartilage homeostasis and OA pathogenesis.