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Preparation and Friction Force Microscopy Measurements of Immiscible, Opposing Polymer Brushes
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Nanoparticle Adsorption on Antifouling Polymer Brushes.

Christina M Zeuthen1,2, Ali Shahrokhtash1, Duncan S Sutherland1

  • 1Interdisciplinary Nanoscience Center , Aarhus University , Gustav Wieds vej 14 , 8200 Aarhus N , Denmark.

Langmuir : the ACS Journal of Surfaces and Colloids
|October 23, 2019
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Summary

This study shows how to immobilize nanoparticles (NPs) on antifouling polymer brushes, a new method for studying NP-protein interactions and applications in biotechnology.

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Area of Science:

  • Biotechnology
  • Materials Science
  • Surface Chemistry

Background:

  • Antifouling polymer brushes are crucial for surface functionalization, preventing unwanted protein and cell adhesion.
  • Existing research focuses on cell/protein interactions with these brushes, neglecting nanoparticle immobilization.
  • Immobilizing nanoparticles (NPs) on antifouling surfaces presents a novel research and application avenue.

Purpose of the Study:

  • To demonstrate the adsorption of pristine and protein-coated nanoparticles onto antifouling polymer brush coatings.
  • To investigate the influence of ionic strength, substrate material, and NP surface charge on NP adsorption.
  • To explore the potential of this method for studying nanoparticle-protein interactions, like the protein corona.

Main Methods:

  • Utilized poly-l-lysine-graft-poly(ethylene glycol) (PLL-g-PEG) and methoxy PEG-thiol to create antifouling polymer brush coatings.
  • Investigated the adsorption of various nanoparticles (polystyrene, gold, carbon black, silica) onto these coatings.
  • Employed surface plasmon resonance and fluorescence imaging to study NP adsorption and interactions.

Main Results:

  • Demonstrated successful adsorption of both pristine and protein-coated nanoparticles onto PLL-g-PEG brush surfaces.
  • Identified ionic strength, substrate material, and NP surface charge as key factors influencing adsorption.
  • Confirmed that polystyrene, gold, carbon black, and silica NPs can adsorb onto the antifouling surfaces.

Conclusions:

  • Developed a novel method for self-assembling nanoparticles onto antifouling polymer brush surfaces.
  • This platform facilitates the study of nanoparticle-protein interactions, including protein corona formation.
  • Offers a versatile platform for scientific research and biotechnological applications.