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Published on: October 21, 2016
Preparation and characterization of carboxymethyl cashew gum grafted with immobilized antibody for potential
Airis Maria Araújo Melo1, Maria Roniele Felix Oliveira1, Roselayne Ferro Furtado2
1Department of Chemistry, State University of Ceara, 1700 Dr. Silas Munguba Avenue, Fortaleza, CE 60740-903, Brazil.
Abstract:
This report details the design of carboxymethylated cashew gum (CG) as a platform for antibody (Ab) immobilization, which can then be used as a biosensor for bacteria detection. The CG was isolated and characterized, followed by conversion to carboxymethyl cashew gum (CMCG). The CMCG film was a viable support for antibody immobilization; it was electrodeposited on gold surface using the cyclic voltammetry technique, applying a potential sweep from -1.0 V to 1.3 V with a scan rate of 50 mV s-1 and 10 scans. The COOH groups on the surface of the film were critical in promoting Ab bonding. The immobilization of the Ab was mediated by protein A (PrA) for recognition of the antigen. Voltammetry studies were used to monitor the antibody immobilization. Finally, the analytical response of the CMCG-PrA-Ab system was evaluated with the chronoamperometry technique and was found to detect Salmonella Typhimurium bacteria rapidly and efficiently.
