Related Experiment Video

Updated: Jan 5, 2026

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.5K

SMARCD1 is a transcriptional target of specific non-hotspot mutant p53 forms

Raju S R Adduri1,2, Sara A George1,3, Padmavathi Kavadipula1

  • 1Laboratory of Molecular Oncology, Centre for DNA Fingerprinting and Diagnostics, Hyderabad, Telangana, India.

Journal of Cellular Physiology
|October 23, 2019
PubMed

Insights

Mutant TP53 protein can activate oncogenes, including SMARCD1, promoting head and neck squamous cell carcinoma (HNSCC) growth and migration. Targeting SMARCD1 may offer a new therapeutic strategy for HNSCC patients with TP53 mutations.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The TP53 tumor suppressor gene, when mutated, can gain oncogenic functions.
  • Specific TP53 missense mutations in the DNA binding domain can lead to transcriptional activation of oncogenes.

Purpose of the Study:

  • To investigate the role of SMARCD1 in head and neck squamous cell carcinoma (HNSCC).
  • To determine if mutant TP53 (p53) activates SMARCD1 expression.
  • To assess the impact of SMARCD1 on HNSCC tumorigenic properties and patient survival.

Main Methods:

  • Microarray-based messenger RNA profiling of oral tongue squamous cell carcinoma (SCCOT).
  • Validation using The Cancer Genome Atlas (TCGA) datasets and additional tongue cancer samples.
  • SMARCD1 knockdown experiments in HNSCC cell lines.
  • Correlation analysis between SMARCD1 expression and patient survival.

Main Results:

  • SMARCD1 was significantly elevated in SCCOT samples with p53 nuclear stabilization.
  • Mutant p53 was confirmed to activate SMARCD1 transcription.
  • SMARCD1 knockdown reduced HNSCC cell viability, colony formation, and migration.
  • Increased SMARCD1 expression correlated with poor survival in HNSCC harboring missense p53 mutations.

Conclusions:

  • SMARCD1 is a novel transcriptional target of mutant p53.
  • Mutant p53-driven SMARCD1 activation contributes to HNSCC progression.
  • SMARCD1 represents a potential therapeutic target in HNSCC with TP53 mutations.
Keywords:
SMARCD1ZMAT3gain of oncogenic functionmutant p53squamous cell carcinoma of the oral tongue

More Related Videos

Yeast As a Chassis for Developing Functional Assays to Study Human P5314:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

10.0K
Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence12:09

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

12

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.6K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.1K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.2K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
8.6K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.9K