Related Experiment Video

Updated: Jan 5, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

18.4K

Nitrite may serve as a combination partner and a biomarker for the anti-cancer activity of RRx-001

Selma Cirrik1, Elif Ugurel2, Ali Cenk Aksu2

  • 1Ordu University, Faculty of Medicine, Department of Physiology, Ordu, Turkey.

Biorheology
|October 24, 2019
PubMed
Abstract

Insights

RRx-001, an anti-cancer immunotherapeutic, can alter red blood cell deformability. Combining RRx-001 with nitrite under hypoxia significantly enhances erythrocyte deformability by increasing nitric oxide production.

Area of Science:

  • Oncology
  • Immunotherapy
  • Hematology

Background:

  • RRx-001 is an anti-cancer immunotherapeutic agent.
  • It enhances tumor sensitivity to chemotherapy through hemoglobin binding and nitrite reductase activity.
  • The study investigates RRx-001's impact on red blood cell (RBC) function.

Purpose of the Study:

  • To examine the effect of RRx-001 on erythrocyte deformability at clinically relevant doses.
  • To assess the role of nitric oxide (NO) in mediating these effects.
  • To explore potential therapeutic strategies involving RRx-001 and nitrite.

Main Methods:

  • Erythrocyte deformability was measured using ektacytometry under normoxic and hypoxic conditions.
  • RRx-001 was tested at various concentrations (1-1000 micro molar).
  • Intracellular NO levels were quantified using DAF-FM-DA, and effects of ODQ and L-NAME were assessed.

Main Results:

  • Higher doses of RRx-001 (100, 1000 micro molar) significantly reduced erythrocyte deformability under hypoxia.
  • Low-dose RRx-001 (20 micro molar) did not alter deformability alone or with ODQ/L-NAME.
  • RRx-001 combined with nitrite significantly increased erythrocyte deformability under hypoxia, correlating with elevated NO production.

Conclusions:

  • Co-administration of RRx-001 and nitrite under hypoxia enhances erythrocyte deformability via increased NO production.
  • Monitoring and supplementing serum nitrite levels in cancer patients treated with RRx-001 may optimize therapeutic efficacy.
  • This suggests a potential adjunctive role for nitrite in RRx-001 cancer immunotherapy.
Keywords:
RRx-001deformabilityerythrocytehypoxianitric oxide

More Related Videos

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method08:25

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

23.0K
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

4.7K

Related Concept Videos

2° Amines to N-Nitrosamines: Reaction with NaNO201:20

2° Amines to N-Nitrosamines: Reaction with NaNO2

Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.
5.3K
Transducer Mechanism: Nuclear Receptors01:31

Transducer Mechanism: Nuclear Receptors

Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
2.3K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.6K