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G-Quadruplex-Forming Aptamers-Characteristics, Applications, and Perspectives.
Carolina Roxo1, Weronika Kotkowiak2, Anna Pasternak3
1Department of Nucleic Acids Bioengineering, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland. croxo@ibch.poznan.pl.
Molecules (Basel, Switzerland)
|October 24, 2019
Summary
G-quadruplexes are unique nucleic acid structures with therapeutic and diagnostic potential. G-rich aptamers, developed using SELEX technology, offer stable, cost-effective alternatives to antibodies for disease treatment.
Area of Science:
- Molecular Biology
- Biochemistry
- Genetics
Background:
- G-quadruplexes are polymorphic nucleic acid structures found in the human genome.
- They play crucial roles in regulating biological processes like replication, transcription, and translation.
- These structures are emerging as significant targets for disease treatment.
Purpose of the Study:
- To review recent advancements in G-quadruplex-based aptamer development.
- To highlight the therapeutic and diagnostic potential of G-quadruplex aptamers.
- To explore G-rich aptamers as alternatives to antibodies.
Main Methods:
- Systematic Evolution of Ligands by Exponential Enrichment (SELEX) technology.
- Characterization of G-quadruplex structures and their aptamers.
- Evaluation of aptamer stability, cellular uptake, and production feasibility.
Main Results:
- SELEX technology has yielded numerous G-rich aptamers targeting various ligands.
- G-quadruplex aptamers exhibit high stability, efficient cellular uptake, and cost-effectiveness.
- These aptamers show promise as therapeutic and diagnostic agents.
Conclusions:
- G-quadruplex aptamers represent a promising class of molecular tools for therapeutic and diagnostic applications.
- Their unique properties make them valuable alternatives to traditional antibody-based approaches.
- Continued research in G-quadruplex aptamer development holds significant potential for advancing medicine.
