Related Experiment Video
Updated: Jan 5, 2026
Sperm Collection of Differential Quality Using Density Gradient Centrifugation
Published on: November 29, 2018
Effect of temperature and time after collection on buck sperm quality
Kirsten Hahn1, Klaus Failing2, Axel Wehrend3
1Equine Hospital, Ludwig-Maximilians-University, Veterinärstraße 13, 80539, Munich, Germany. k.hahn@pferd.vetmed.uni-muenchen.de.
Background:
Different parameters are assessed as part of the semen analysis but a standard protocol for evaluation of goat semen is still missing. The aim of this study was to analyse two different factors affecting buck sperm quality in the post-collection period prior to adding the extender. Here we examined the effects of two handling temperatures (20 °C, 37 °C) and various examination time points (3-30 min) after semen collection.
Results:
Examination time point had a significant influence on raw sperm viability (p < 0.05), motility (p < 0.05) and on semen pH (p < 0.05). The two different handling temperatures had no significant effect on sperm viability (p > 0.05), motility (p > 0.05), with the exception of fast moving sperm (p = 0.04), or on semen pH (p > 0.05).
Conclusion:
Examination time point was identified as factor strongly influencing raw peacock buck semen after collection. Raw goat semen can tolerate room temperatures for at least 10 min without impacting overall semen quality. In order to obtain comparable results, semen samples should always be examined within 10 min after collection.
