Related Experiment Video

Updated: Jan 5, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

8.3K

[Role of the Notch signaling pathway in children with tuberculosis]

Qi-Feng Li1, Xiang-Yan He, Tao Xin

  • 1Xinjiang Institute of Pediatrics, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China. liqiqi521@sina.com.

Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics
|October 24, 2019
PubMed

Insights

Children with tuberculosis show increased Notch1/2 and DLL4 mRNA expression. This suggests the Notch signaling pathway may influence childhood tuberculosis pathogenesis via other targets, requiring further investigation.

Area of Science:

  • Immunology
  • Molecular Biology
  • Pediatrics

Background:

  • The Notch signaling pathway is crucial in immune cell development and function.
  • Tuberculosis (TB) is a significant global health issue, particularly in children.
  • Understanding molecular pathways involved in childhood TB pathogenesis is essential for developing targeted therapies.

Purpose of the Study:

  • To investigate the expression of Notch signaling pathway molecules in children diagnosed with tuberculosis.
  • To explore the potential role of the Notch pathway in the pathogenesis of childhood tuberculosis.

Main Methods:

  • Quantitative real-time PCR was employed to measure mRNA expression levels.
  • Leukocytes from 62 children with TB and 64 healthy controls were analyzed.
  • Expression of Notch receptors (Notch1-4), ligands (Jagged1/2, DLL1/3/4), and downstream genes (Hes1, Hey1) was quantified.

Main Results:

  • Children with TB exhibited significantly higher mRNA expression of Notch1, Notch2, and DLL4 compared to healthy controls (P<0.05).
  • No significant differences in mRNA expression were observed for Notch3/4, Jagged1/2, DLL1/3, Hes1, and Hey1 between the groups (P>0.05).

Conclusions:

  • Elevated Notch1/2 and DLL4 mRNA expression in children with TB indicates potential activation of the Notch pathway.
  • The lack of change in downstream target genes suggests the pathway may influence childhood TB through alternative mechanisms.
  • Further research is warranted to elucidate the precise role of the Notch signaling pathway in childhood tuberculosis pathogenesis.
Abstract

More Related Videos

A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection10:10

A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection

Published on: October 5, 2015

19.4K
Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF107:42

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Published on: February 28, 2025

862

Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.3K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.4K
Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
796
Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
1.3K
Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
516
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
872