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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Using RNA Interference for Purinoceptor Knockdown In Vivo.

Rebeca Padrão Amorim1, Iscia Teresinha Lopes Cendes2, Maria Jose da Silva Fernandes3,4

  • 1Departamento de Neurologia e Neurocirurgia, Disciplina de Neurociência, Universidade Federal de São Paulo-UNIFESP, São Paulo, Brazil.

Methods in Molecular Biology (Clifton, N.J.)
|October 25, 2019
PubMed
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RNA interference (RNAi) silences genes using small interfering RNA (siRNA). This study explored RNAi mechanisms and purinoceptor function in rat brains via siRNA infusion.

Keywords:
BrainHippocampusPurinergic receptorRNAiRat

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Area of Science:

  • Molecular Biology
  • Neuroscience

Background:

  • RNA interference (RNAi) is a biological process for gene silencing.
  • It functions post-transcriptionally, reducing protein production via mRNA degradation or translation inhibition.
  • Small double-stranded RNA (dsRNA) triggers RNAi.

Purpose of the Study:

  • To provide an overview of RNA interference mechanisms.
  • To investigate purinoceptor function in the rat brain using RNAi.

Main Methods:

  • Overview of RNA interference (RNAi) mechanisms.
  • Nonviral infusion of short small interfering RNA (siRNA) into the rat ventricular system.

Main Results:

  • The study provides a foundational understanding of RNAi pathways.
  • It demonstrates the application of siRNA for studying specific receptors in vivo.

Conclusions:

  • RNA interference is a versatile tool for gene silencing.
  • siRNA delivery to the brain is feasible for studying neurological targets like purinoceptors.