Flow Cytometry of Membrane Purinoreceptors
Nicole Schwarz1, Marten Junge1, Friedrich Haag1
1Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|October 25, 2019
Summary
This study details flow cytometry protocols for measuring purinoreceptor expression and activity on living cells. These methods help analyze purinergic signaling pathways involving receptors like P2X and P2Y.
Area of Science:
- Cell biology
- Biochemistry
- Pharmacology
Background:
- Mammalian cells utilize purinoreceptors to sense extracellular nucleotides and metabolites.
- Purinoreceptors include ionotropic P2X and metabotropic P1/P2Y receptors on the cell surface.
- These receptors play crucial roles in various physiological processes.
Purpose of the Study:
- To provide standardized protocols for monitoring purinoreceptor expression and activity.
- To enable the study of purinergic signaling in living cells using flow cytometry.
Main Methods:
- Flow cytometry techniques are described for analyzing cell surface purinoreceptors.
- Protocols focus on quantifying receptor presence and functional activity.
Main Results:
- The chapter offers detailed, reproducible methods for purinoreceptor analysis.
- Demonstrates the utility of flow cytometry for studying purinergic signaling.
Conclusions:
- Flow cytometry provides a powerful tool for assessing purinoreceptor dynamics in real-time.
- These protocols facilitate research into the roles of purinoreceptors in health and disease.
