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Updated: Jan 5, 2026
Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes
Published on: February 14, 2025
Determining transaminase activity in bacterial libraries by time-lapse imaging
Carlos J C Rodrigues1, João M Sanches2, Carla C C R de Carvalho1
1iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal. ccarvalho@tecnico.ulisboa.pt.
Abstract:
Transaminase activity was determined by time-lapse imaging using a colourimetric reaction and image analysis. A correlation between the benzaldehyde conversion and relative luminance was determined, allowing the identification of the most promising biocatalysts, the determination of kinetic parameters, and the assessment of the effect of the substrate concentration on activity.
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