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A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles
Published on: March 20, 2019
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High-throughput single nanoparticle detection using a feed-through channel-integrated nanopore.
Makusu Tsutsui1, Tomoko Yamazaki1, Kenji Tatematsu1
1The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan. tsutsui@sanken.osaka-u.ac.jp skuroda@sanken.osaka-u.ac.jp kawai@sanken.osaka-u.ac.jp.
Nanoscale
|October 25, 2019
Summary
This study introduces a novel microfluidics and multipore electrophoresis system for highly efficient single-nanoparticle detection. This technology enhances nanopore sensor sensitivity for detecting biosamples like influenza viruses in real-world conditions.
Area of Science:
- Nanotechnology
- Biophysics
- Analytical Chemistry
Background:
- Solid-state nanopore sensors offer high sensitivity but suffer from low detection efficiency.
- Efficiently transferring target analytes into the nanoscale sensing zone remains a challenge.
Purpose of the Study:
- To develop a high-throughput single-nanoparticle detection technology.
- To overcome the limitations of low detection efficiency in current nanopore sensing methods.
Main Methods:
- Integration of microfluidics control and multipore electrophoresis in nanopore-in-channel units.
- Utilizing a silicon nitride (Si3N4) membrane with integrated nanopores.
- Employing hydropressure regulation for controlled mass transport of biosamples.
Main Results:
- Demonstrated a proof-of-concept for influenza virus detection.
- Achieved selective detection of charged objects in physiological media like human saliva.
- Enabled continuous feeding of biosamples into the nanopore's electric field via a feed-through mechanism.
Conclusions:
- The developed nanostructure significantly enhances detection efficiency for nanopore sensors.
- This integrated strategy offers a versatile platform for ultrasensitive bioanalytical tools.
- The technology shows promise for practical applications in detecting various analytes from cells to polynucleotides.
