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Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva
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Dose-dependent green tea effect on decrease of inflammation in human oral gingival epithelial keratinocytes: in vitro

Ana Hagiu1, Thomas Attin1, Patrick R Schmidlin1,2

  • 1Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.

Clinical Oral Investigations
|October 26, 2019
PubMed
Summary

Green tea extract (GTE) reduces inflammation and enhances wound healing in oral keratinocytes by decreasing inflammatory markers and boosting cell migration. GTE activates the Nrf2/HO-1 pathway, showing potential for oral anti-inflammatory treatments.

Keywords:
Green tea extractHO-1InflammationLipopolysaccharideNrf2Oral rinse

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Area of Science:

  • Oral biology
  • Inflammation research
  • Natural product therapeutics

Background:

  • Oral inflammatory diseases pose significant health challenges.
  • Green tea extract (GTE) is recognized for its potential therapeutic properties.

Purpose of the Study:

  • To investigate the anti-inflammatory and wound healing effects of GTE on human gingival epithelial keratinocytes (HGEK).
  • To analyze the impact of GTE on lipopolysaccharide (LPS)-induced inflammation and cell migration in HGEK.
  • To elucidate the molecular mechanisms underlying GTE's effects, including the Nrf2/HO-1 pathway.

Main Methods:

  • Cell viability was assessed using MTT assay.
  • Inflammatory gene and protein expression (IL-1β, IL-6, TNFα) were measured by RT-PCR and ELISA.
  • Scratch wound healing assay evaluated cell migration.
  • Nrf2/HO-1 pathway activation was investigated.

Main Results:

  • GTE significantly enhanced HGEK viability at concentrations of 2.5, 5, and 10 mg/ml.
  • GTE treatment dose-dependently decreased the gene and protein expression of IL-1β, IL-6, and TNFα.
  • GTE increased cell migration in the scratch wound healing assay.

Conclusions:

  • GTE exhibits anti-inflammatory properties by reducing key inflammatory markers in HGEK.
  • GTE promotes wound healing by enhancing cell migration.
  • The anti-inflammatory mechanism involves the activation of the Nrf2/HO-1 pathway.
  • GTE holds potential as an ingredient in oral rinses for managing oral inflammatory conditions.