Related Experiment Video

Updated: Jan 5, 2026

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.3K

Long non-coding RNA MIR205HG regulates KRT17 and tumor processes in cervical cancer via interaction with SRSF1

Mingli Dong1, Zhennan Dong2, Xinyu Zhu3

  • 1Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing 100853, China.

Experimental and Molecular Pathology
|October 27, 2019
PubMed

Insights

Long non-coding RNA MIR205HG is upregulated in cervical cancer, promoting cancer cell proliferation and migration by regulating KRT17 expression via SRSF1. This finding offers a potential new therapeutic target for cervical cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Aberrant expression of long non-coding RNAs (lncRNAs) is implicated in various cancers.
  • The specific role and regulatory mechanisms of MIR205HG in cervical cancer remain largely unexplored.

Purpose of the Study:

  • To investigate the expression pattern and functional role of MIR205HG in cervical cancer.
  • To elucidate the underlying molecular mechanism by which MIR205HG influences cervical cancer progression.

Main Methods:

  • Quantitative real-time PCR to assess MIR205HG and KRT17 expression levels.
  • Cell proliferation, migration, and apoptosis assays following KRT17 or MIR205HG manipulation.
  • Western blotting and RNA immunoprecipitation assays to determine the interaction between MIR205HG, SRSF1, and KRT17.

Main Results:

  • MIR205HG and KRT17 were found to be significantly upregulated in cervical cancer tissues and cell lines.
  • Silencing KRT17 inhibited cervical cancer cell proliferation and migration while promoting apoptosis.
  • MIR205HG depletion suppressed cancer progression, and rescue experiments confirmed MIR205HG targets SRSF1 to upregulate KRT17, impacting cell proliferation, migration, and apoptosis.

Conclusions:

  • MIR205HG acts as an oncogenic lncRNA in cervical cancer by modulating the MIR205HG/SRSF1/KRT17 axis.
  • This pathway influences key cellular processes including proliferation, migration, and apoptosis in cervical cancer.
  • MIR205HG represents a potential therapeutic target for cervical cancer intervention.
Keywords:
Cervical cancerKRT17MIR205HGSRSF1

More Related Videos

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells11:11

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells

Published on: April 25, 2018

8.6K
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.8K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.7K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.4K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.7K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.8K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.3K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.2K