Connections between 3' end processing and DNA damage response: Ten years later.

Area of Science:

• Molecular Biology
• Genetics
• Biochemistry

Background:

• The DNA damage response (DDR) is crucial for maintaining genomic stability.
• Gene expression regulation in DDR involves transcriptional control and mRNA turnover.
• The 3' end processing machinery influences mRNA stability and gene-specific DDR.

Purpose of the Study:

• To review recent mechanistic insights into the interplay between 3' end processing and DDR.
• To highlight the roles of alternative polyadenylation, microRNA, and deadenylation in DDR.
• To discuss the implications of dysregulated 3' end processing in DDR and human diseases.

Main Methods:

• Literature review of recent mechanistic studies.
• Focus on alternative polyadenylation mechanisms.
• Analysis of microRNA and RNA-binding protein roles in mRNA deadenylation within DDR.

Main Results:

• 3' end processing directly impacts mRNA stability and availability for DDR genes.
• Alternative polyadenylation influences transcript isoforms involved in DNA repair.
• MicroRNA-mediated deadenylation and RNA-binding proteins fine-tune DDR gene expression.

Conclusions:

• 3' end processing is a critical regulatory layer in the DDR.
• Dysregulation of these RNA processing events contributes to human diseases.
• Targeting 3' end processing pathways may offer therapeutic strategies for DDR-related disorders.