Immunoexpression of Snail, Twist1 and Slug in clear cell renal cell carcinoma
Bianca Cătălina Andreiana1, Alex Emilian Stepan, Oana Taisescu
1Department of Pathology, University of Medicine and Pharmacy of Craiova, Romania; csimionescu2004@yahoo.com, astepan76@yahoo.com.
Abstract:
Clear cell renal cell carcinomas (ccRCC) represent about 80% of the malignant neoplasia with this localization. Snail, Twist1 and Slug are transcription factors and play a central role in the epithelial-mesenchymal transition (EMT), which is involved in the progression of renal cell carcinoma (RCC). In this study, we analyzed the immunoexpression of these transcription factors in 50 cases of ccRCC, in relation to histopathological aggressiveness parameters of the lesions. The results indicated the association of Snail and Twist1 expression with high Fuhrman grade, as well as the association of Slug expression with low Fuhrman grade. The immunoexpression of Snail and Twist1 was significantly superior for advanced stages and Slug was overexpressed in early stages of ccRCC. Our study supports the usefulness of the Snail, Twist1 and Slug expression for the appreciation of aggressiveness in ccRCC, the panel being attractive for targeted therapy.
Insights
This study shows that Snail, Twist1, and Slug transcription factors indicate clear cell renal cell carcinoma (ccRCC) aggressiveness. Their expression levels correlate with tumor grade and stage, aiding in predicting ccRCC progression.
Area of Science:
- Oncology
- Molecular Biology
- Pathology
Background:
- Clear cell renal cell carcinoma (ccRCC) is the most common kidney cancer.
- Epithelial-mesenchymal transition (EMT) is crucial in renal cell carcinoma (RCC) progression.
- Snail, Twist1, and Slug are key transcription factors regulating EMT.
Purpose of the Study:
- To analyze the immunoexpression of Snail, Twist1, and Slug in ccRCC.
- To correlate transcription factor expression with histopathological aggressiveness parameters.
- To evaluate their potential as biomarkers for ccRCC progression and targeted therapy.
Main Methods:
- Immunohistochemical analysis of Snail, Twist1, and Slug expression.
- Evaluation of 50 ccRCC cases.
- Correlation with Fuhrman grade and tumor stage.
Main Results:
- Snail and Twist1 expression associated with high Fuhrman grade and advanced stages.
- Slug expression associated with low Fuhrman grade and early stages.
- Differential expression patterns observed for ccRCC progression.
Conclusions:
- Snail, Twist1, and Slug expression are useful indicators of ccRCC aggressiveness.
- These transcription factors can help assess tumor progression.
- The panel shows potential for guiding targeted therapy in ccRCC.
