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Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
Published on: May 23, 2025
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CRISPR: A Screener's Guide
Carlos le Sage1, Steffen Lawo1, Benedict C S Cross1
1Horizon Discovery, Waterbeach, Cambridge, UK.
SLAS Discovery : Advancing Life Sciences R & D
|October 30, 2019
Summary
CRISPR gene editing is revolutionizing drug discovery by enabling large-scale functional genomic screening for precision medicines. This technology enhances target identification and validation, impacting pharmaceutical research and development.
Area of Science:
- Biotechnology
- Genomics
- Drug Discovery
Background:
- CRISPR-Cas9 systems have significantly advanced gene editing technologies.
- Gene editing is increasingly adopted in pharmaceutical and biotechnology research.
- Functional genomic screening links biological phenomena to underlying genes.
Purpose of the Study:
- To review the role of CRISPR screening technologies in the pharmaceutical industry.
- To explore the application of CRISPR at an industrial scale for target identification and validation.
- To examine the use of CRISPR in understanding drug resistance and sensitivity during development.
Main Methods:
- Utilizing CRISPR-Cas9 systems for large-scale functional genomic screening.
- Applying gene editing for robust target identification and validation.
- Employing CRISPR-based screens in the drug development pipeline.
Main Results:
- CRISPR screening provides more robust datasets for target identification and validation.
- Functional genomic strategies are crucial for linking biological observations to genes.
- CRISPR approaches aid in understanding drug resistance and sensitivity.
Conclusions:
- CRISPR screening technologies are a developing and evolving platform within the pharmaceutical industry.
- The industrial-scale application of CRISPR enhances precision medicine development.
- Future steps involve further integration and advancement of CRISPR screening platforms.
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