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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Updated: Jan 5, 2026

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
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Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

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CRISPR: A Screener's Guide.

Carlos le Sage1, Steffen Lawo1, Benedict C S Cross1

  • 1Horizon Discovery, Waterbeach, Cambridge, UK.

SLAS Discovery : Advancing Life Sciences R & D
|October 30, 2019
PubMed
Summary
This summary is machine-generated.

CRISPR gene editing is revolutionizing drug discovery by enabling large-scale functional genomic screening for precision medicines. This technology enhances target identification and validation, impacting pharmaceutical research and development.

Keywords:
CRISPRRNAicancer and cancer drugscell-based assaysgene editinghigh-content screeningin vivo screeningshRNA

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Area of Science:

  • Biotechnology
  • Genomics
  • Drug Discovery

Background:

  • CRISPR-Cas9 systems have significantly advanced gene editing technologies.
  • Gene editing is increasingly adopted in pharmaceutical and biotechnology research.
  • Functional genomic screening links biological phenomena to underlying genes.

Purpose of the Study:

  • To review the role of CRISPR screening technologies in the pharmaceutical industry.
  • To explore the application of CRISPR at an industrial scale for target identification and validation.
  • To examine the use of CRISPR in understanding drug resistance and sensitivity during development.

Main Methods:

  • Utilizing CRISPR-Cas9 systems for large-scale functional genomic screening.
  • Applying gene editing for robust target identification and validation.
  • Employing CRISPR-based screens in the drug development pipeline.

Main Results:

  • CRISPR screening provides more robust datasets for target identification and validation.
  • Functional genomic strategies are crucial for linking biological observations to genes.
  • CRISPR approaches aid in understanding drug resistance and sensitivity.

Conclusions:

  • CRISPR screening technologies are a developing and evolving platform within the pharmaceutical industry.
  • The industrial-scale application of CRISPR enhances precision medicine development.
  • Future steps involve further integration and advancement of CRISPR screening platforms.