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How oncogenic mutations activate human MAP kinase 1 (MEK1): a molecular dynamics simulation study.

Ye Liu1, Jingxuan Zhu1, Xiaoqing Guo1

  • 1Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Science, Jilin University, Changchun, China.

Journal of Biomolecular Structure & Dynamics
|October 30, 2019
PubMed
Summary

Activating mutations in MAP kinase 1 (MEK1) promote cancer by altering its structure and reducing inhibitor effectiveness. This study reveals how these mutations transform MEK1 into its active form, driving cell proliferation.

Keywords:
MAP kinase 1 (MEK1)MAPK pathwayMM-PBSAcancerconformational changemolecular dynamics simulation

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • The mitogen-activated protein kinase (MAPK) pathway is constitutively activated in ~30% of human cancers.
  • MAP kinase 1 (MEK1) is a key component of the MAPK pathway, and its mutations can lead to oncogenic cell proliferation and transformation.

Purpose of the Study:

  • To elucidate the molecular mechanisms by which mutations activate MEK1.
  • To understand how MEK1 mutations affect its interaction with inhibitors.

Main Methods:

  • Eight molecular dynamics simulations.
  • Molecular mechanics Poisson-Boltzmann surface area (MM-PBSA) calculations.
  • Protein structure network analysis.
  • Hydrogen bond analysis.

Main Results:

  • Activating mutations disrupt the interaction between MEK1's activation segments and C-helix, converting it to an active form.
  • Mutations decrease MEK1's binding affinity for inhibitors, reducing their efficacy.
  • Specific mutations alter the Pro-rich loop, potentially stabilizing MEK1-ligand interactions through increased hydrophobic residue exposure.

Conclusions:

  • The study provides theoretical evidence for the mechanism of MEK1 activation by mutations in cancer.
  • Findings offer insights into MEK1's role in oncogenesis and potential therapeutic strategies.