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Multiple Synthetic Routes to the Mini-Protein Omomyc and Coiled-Coil Domain Truncations.

Zachary Z Brown1, Claudio Mapelli1, Iman Farasat2

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The Journal of Organic Chemistry
|October 30, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed advanced peptide synthesis methods to create Omomyc, a mini-protein targeting the "undruggable" Myc oncoprotein. This potent mini-protein effectively binds DNA and inhibits cancer cell proliferation, offering a promising new therapeutic strategy.

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Area of Science:

  • Molecular Biology
  • Medicinal Chemistry
  • Cancer Research

Background:

  • The Myc transcription factor is a critical regulator of cell growth and a key driver in numerous cancers.
  • Myc's role in cancer makes it a highly desirable but challenging therapeutic target, often referred to as "undruggable".

Purpose of the Study:

  • To develop and evaluate synthesis strategies for Omomyc, a mini-protein inhibitor of Myc.
  • To explore the structure-activity relationships (SAR) of Omomyc for potential cancer therapies.

Main Methods:

  • Linear Fmoc solid-phase peptide synthesis (SPPS) with specialized techniques for challenging sequences.
  • Native chemical ligation for assembling smaller peptide fragments into the full-length Omomyc.
  • High-throughput bacterial expression and assay platform for rapid mutagenesis and screening.

Main Results:

  • Successfully synthesized Omomyc and analogues in gram quantities using a combination of advanced peptide synthesis techniques.
  • Demonstrated potent DNA binding to the E-box element, crucial for Myc-mediated transcriptional activation.
  • Confirmed cell permeability and significant inhibition of proliferation in Myc-dependent cancer cell lines.
  • Investigated Omomyc's covalent homodimerization via disulfide bonds and identified truncations of the coiled-coil region that preserve activity.

Conclusions:

  • Advanced peptide synthesis enables the tractable SAR exploration of challenging mini-protein modalities like Omomyc.
  • Omomyc and its analogues are potent inhibitors of Myc transcriptional activity and show promise as anti-cancer agents.
  • The development of efficient synthesis routes facilitates the progression of Omomyc as a potential therapeutic candidate.