Hydrogenophaga borbori sp. nov., isolated from activated sludge

A novel Gram-stain-negative, yellowish-pigmented bacterial strain, designated LA-38^T, was isolated from activated sludge of wastewater treatment plants in Hanam city, South Korea. Cell of LA-38^T were rod-shaped, aerobic, motile and non-spore-forming. In phylogenetic analyses based on 16S rRNA genes, LA-38^T clustered with species of the genus Hydrogenophaga and appeared closely related to Hydrogenophaga intermedia DSM 5680^T (99.2 % similarity), Hydrogenophaga palleronii DSM 63^T (98.2 %), Hydrogrenophaga laconesensis KCTC 42478^T (98.1 %), Hydrogenophaga. atypica DSM 15342^T (98.1 %), Hydrogenophaga defluvii DSM 15341^T (98.0 %) and Hydrogenophaga taeniospiralis DSM 2082^T (97.2 %). The average nucleotide identities between LA-38^T and the closely related strains were 79.3-88.5 %, indicating that LA-38^T represents a novel species of the genus Hydrogenophaga. The DNA G+C content of the genomic DNA was 69.9 mol% and ubiquinone Q-8 was the predominant respiratory quinone. The major cellular fatty acids (>5 %) were C_16 : 0, cyclo-C_19 : 0, C_16 : 1ω7c and/or C_16 : 1ω6c (summed feature 3), and C_18 : 1ω7c and/or C_18 : 1ω6c (summed feature 8). The major polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine, the major polyamines were 2-hydroxyputrescine and putrescine. ANI calculation, physiological and biochemical characteristics indicated that LA-38^T represents a novel species of the genus Hydrogenophaga, for which the name Hydrogenophaga borbori sp. nov. is proposed. The type strain is LA-38^T (=KACC 19730^T=LMG 30805^T).