Chryseobacterium binzhouense sp. nov., isolated from activated sludge

Area of Science:

• Microbiology
• Bacteriology
• Environmental Microbiology

Background:

• The genus Chryseobacterium comprises Gram-negative bacteria with diverse environmental roles.
• Wastewater treatment plants harbor a wide array of microbial communities, including potential novel species.
• Characterization of novel bacteria is crucial for understanding microbial diversity and ecological functions.

Purpose of the Study:

• To isolate and characterize a novel bacterium from activated sludge.
• To determine the taxonomic and phylogenetic placement of the novel isolate.
• To propose a new species within the genus Chryseobacterium.

Main Methods:

• Isolation and cultivation of bacteria from activated sludge.
• Phenotypic characterization, including growth conditions and biochemical tests.
• Phylogenetic analysis using 16S rRNA gene sequencing.
• Genomic analysis, including genome size, G+C content, ANI, and dDDH values.
• Chemotaxonomic analysis of major fatty acids and polar lipids.

Main Results:

• A golden-pigmented, aerobic, Gram-negative bacterium, strain lm2^T, was isolated.
• Phylogenetic analysis placed strain lm2^T within the genus Chryseobacterium, with 97.1% 16S rRNA gene similarity to Chryseobacterium echinoideorum.
• Genomic data (ANI 87.8%, dDDH 34.7%) and chemotaxonomic profiles supported its distinction from known species.
• Optimal growth occurred at 30°C, 0-2.0% NaCl, and pH 7.0-7.5.

Conclusions:

• Strain lm2^T represents a novel species of the genus Chryseobacterium.
• The proposed name for the novel species is Chryseobacterium binzhouense.
• The type strain is lm2^T (=KCTC 72529^T=CCTCC AB2019126^T).