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Isolation and characterization of the Staphylococcus aureus bacteriophage vB_SauS_SA2

Jia Wang1, Feiyang Zhao1, Huzhi Sun2

  • 1Qingdao Agricultural University, College of Veterinary Medicine, Shandong 266109, China.

AIMS Microbiology
|October 31, 2019
PubMed

Insights

A novel bacteriophage, SA2, was isolated to infect Staphylococcus aureus. Its unique genome and characteristics propose a new Siphoviridae genus, SA2likevirus.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Staphylococcus aureus is a significant human pathogen.
  • Bacteriophages are viruses that infect bacteria and are potential therapeutic agents.
  • Characterization of novel bacteriophages is crucial for understanding phage diversity and applications.

Purpose of the Study:

  • To isolate and characterize a novel bacteriophage infecting Staphylococcus aureus.
  • To analyze the genomic features of the isolated phage.
  • To determine the phylogenetic relationship of the phage and propose a new genus.

Main Methods:

  • Isolation and purification of bacteriophage SA2.
  • Determination of latent period and burst size.
  • Genome sequencing and bioinformatic analysis.
  • Phylogenetic analysis and comparison with existing phage databases.

Main Results:

  • Isolation of bacteriophage SA2 with a latent period of 10 min and burst size of 293 PFU/infected cell.
  • Characterization of a double-stranded DNA genome (89,055 bp, 31.9% G+C content) with 130 ORFs, including 18 unique ones.
  • Low genomic similarity to known phages, with no identified virulence genes.
  • Proposal of a new Siphoviridae genus, 'SA2likevirus', based on unique genomic features.

Conclusions:

  • Phage SA2 is a novel bacteriophage with unique genomic characteristics.
  • The discovery of SA2 and its proposed genus expands the known diversity within the Siphoviridae family.
  • SA2 represents a potential candidate for phage therapy against Staphylococcus aureus infections.
Keywords:
Bacteriophage vB_SauS_SA2Genome analysisStaphylococcus aureus

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