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Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

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Updated: Jan 4, 2026

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Published on: May 24, 2019

Progress in Microfluidics-Based Exosome Separation and Detection Technologies for Diagnostic Applications.

Shujing Lin¹, Zixian Yu¹, Di Chen¹

¹School of Electronic Information and Electrical Engineering, Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument, Key Laboratory for Thin Film and Microfabrication Technology of Ministry of Education, Shanghai Jiao Tong University, Shanghai, 200240, China.

Small (Weinheim an Der Bergstrasse, Germany)

|October 31, 2019

Summary

Exosomes are key to cell communication and disease, making them vital diagnostic biomarkers. This study reviews microfluidic techniques for efficient exosome separation and detection, crucial for disease diagnostics.

Keywords:

exosome detection exosome isolation exosome separation microfluidic chips

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An Innovative Method for Exosome Quantification and Size Measurement

An Innovative Method for Exosome Quantification and Size Measurement

Published on: January 17, 2015

Area of Science:

Biotechnology
Nanotechnology
Molecular Biology

Background:

Exosomes, secreted by cells and found in body fluids, are crucial for intercellular communication and disease development.
Their role in disease pathogenesis makes exosomes promising biomarkers for early diagnosis.
Current separation and detection methods face challenges in purity, throughput, sensitivity, and specificity.

Purpose of the Study:

To provide an overview of conventional and microfluidics-based techniques for exosome separation and detection.
To compare the advantages and disadvantages of these methods for diagnostic applications.
To highlight the potential of integrated microfluidic systems for exosome analysis.

Main Methods:

Review of existing literature on exosome separation and detection techniques.
Focus on conventional methods (e.g., ultracentrifugation, precipitation) and their limitations.
Detailed examination of microfluidics-based approaches for integrated exosome analysis.

Main Results:

Conventional methods often require separate, complex, time-consuming, and costly procedures.
Microfluidics offers integrated platforms for simultaneous exosome separation and detection.
Microfluidic devices show potential for high-purity, high-throughput, rapid, sensitive, and specific exosome analysis.

Conclusions:

Integrated microfluidic systems represent a significant advancement for exosome-based diagnostics.
Further development of microfluidics is essential to overcome current limitations in exosome analysis.
Microfluidics holds promise for streamlined, cost-effective, and efficient disease biomarker discovery.