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Polymer-Salt Aqueous Two-Phase System (ATPS) Micro-Droplets for Cell Encapsulation.

Mohammad Mastiani¹, Negar Firoozi², Nicholas Petrozzi²

¹Center for Biosignatures Discovery Automation, School of Electrical, Computer and Energy Engineering, Arizona State University, Tempe, AZ 85287, USA.

Scientific Reports

|October 31, 2019

Summary

This study introduces passive generation of salt-based aqueous two-phase system (ATPS) microdroplets for biocompatible cell encapsulation. The polymer/salt ATPS platform demonstrated adequate cell survival, offering a promising method for bioengineering applications.

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Area of Science:

Biomedical Engineering
Biofluidics
Cell Encapsulation Technologies

Background:

Aqueous two-phase systems (ATPS) offer biocompatibility for biological separation and encapsulation.
Passive generation of salt-based ATPS microdroplets has not been previously demonstrated.

Purpose of the Study:

To demonstrate the passive generation of salt-based ATPS microdroplets.
To evaluate the biocompatibility of the polymer/salt ATPS for cell encapsulation.
To assess the potential of this system for encapsulating human umbilical vein endothelial cells (HUVECs).

Main Methods:

Utilized a flow-focusing geometry for droplet generation.
Investigated both polymer/polymer (polyethylene glycol/dextran) and polymer/salt (PEG/Magnesium sulfate) ATPS.
Performed cell viability tests on Magnesium sulfate solutions with HUVECs.

Main Results:

Achieved passive generation of uniform microdroplets (20-60 μm) using the PEG/Magnesium sulfate system.
Demonstrated adequate cell survival for HUVECs encapsulated in the PEG/salt ATPS.
Confirmed the biocompatibility of the polymer/salt ATPS for cell encapsulation.

Conclusions:

The polymer/salt ATPS is a viable all-aqueous platform for generating uniform microdroplets.
This system shows significant potential for biocompatible cell encapsulation in biomedical applications.
Further research can explore diverse cell types and advanced encapsulation strategies.