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Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

Published on: February 15, 2019

Experimental datasets on processed eggshell membrane powder for wound healing.

Tamer A E Ahmed^1,2, Henri-Pierre Suso³, Maxwell T Hincke^2,4

¹Medical Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technology Applications (SRTA-City), Alexandria, Egypt.

|November 1, 2019

Summary

Processed eggshell membrane powder (PEP) shows promise as a novel wound healing product. This study details PEP

Keywords:

Histological scoring Processed eggshell membrane powder Proteomics Wound healing

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Area of Science:

Biomaterials Science
Regenerative Medicine
Proteomics

Background:

Eggshell (ES) and eggshell membrane (ESM) are abundant agricultural byproducts.
Valorization of ES and ESM waste presents opportunities for sustainable biomaterial development.
Previous research explored ES waste for value-added applications.

Purpose of the Study:

To characterize processed eggshell membrane powder (PEP) using proteomics.
To evaluate the efficacy of PEP as a wound healing agent in a mouse model.

Main Methods:

PEP biomaterial characterized by proteomics under various extraction conditions (LDS, urea/ammonium bicarbonate, 3-MPA, NaOH/DMSO).
Proteomic analysis determined the relative abundance of PEP protein constituents.
Wound healing efficacy assessed using the mouse excisional wound splinting model and histological scoring.

Main Results:

Proteomic analysis provided insights into PEP's protein composition.
The study generated datasets on PEP characterization and in vivo wound healing evaluation.
Histological scoring confirmed the efficacy of PEP in promoting wound healing.

Conclusions:

Processed eggshell membrane powder (PEP) is a viable biomaterial for wound healing applications.
Proteomic characterization is crucial for understanding the functional properties of PEP.
PEP demonstrates potential as a cost-effective and sustainable wound healing product.