Related Experiment Video
Updated: Jan 4, 2026
09:37
An Integrated Approach for Microprotein Identification and Sequence Analysis
Published on: July 12, 2022
3.9K
Data for positive selection test and co-evolutionary analysis on mammalian cereblon
Wataru Onodera1, Toru Asahi1,2, Naoya Sawamura1,2
1Faculty of Science and Engineering, Waseda University, TWIns, 2-2 Wakamatsu, Shinjuku, Tokyo, 162-8480, Japan.
Data in Brief
|November 1, 2019
Summary
Molecular evolutionary analyses reveal positive selection in Cereblon (CRBN), a key protein in the CRL4 E3 ubiquitin ligase complex. This suggests CRBN
Area of Science:
- Molecular Biology
- Evolutionary Biology
- Biochemistry
Background:
- Cereblon (CRBN) functions as a substrate recognition subunit of the CRL4 E3 ubiquitin ligase complex.
- CRBN plays roles in energy metabolism, ion-channel activation, and cellular stress response.
- Protein interactions suggest selective pressure leading to functional divergence in CRBN.
Purpose of the Study:
- To investigate molecular evolutionary patterns in mammalian Cereblon (CRBN).
- To analyze selective pressures and co-evolutionary relationships of CRBN.
Main Methods:
- Multiple sequence alignment of CRBN datasets.
- dN/dS calculations to detect positive selection.
- Co-evolutionary analysis using phylogenetic tree comparisons and correlation coefficients.
Main Results:
- Evidence of positive selection in CRBN was identified through dN/dS calculations.
- Co-evolutionary analysis indicated similar evolutionary trajectories between CRBN and related proteins.
- These findings suggest modifications in CRBN's E3 ubiquitin ligase activity and binding efficiency.
Conclusions:
- Positive selection has shaped the evolution of CRBN.
- CRBN's functional divergence is influenced by evolutionary pressures on its interacting surfaces.
- Understanding CRBN evolution provides insights into its diverse cellular roles.
Related Concept Videos
01:54Convergent Evolution
31.3K
Evolution shapes the features of organisms over time, ensuring that they are suited for the environments in which they live. Sometimes, selection pressure leads to the rise of similar but unrelated adaptations in organisms with no recent common ancestors, a process known as convergent evolution.
31.3K
02:31Synteny and Evolution
3.7K
John H. Renwick first coined the term “synteny” in 1971, which refers to the genes present on the same chromosomes, even if they are not genetically linked. The species with common ancestry tend to show conserved syntenic regions. Therefore, the concept of synteny is nowadays used to describe the evolutionary relationship between species.
Around 80 million years ago, the human and mice lineages diverged from the common ancestor. During the course of evolution, the ancestral...
Around 80 million years ago, the human and mice lineages diverged from the common ancestor. During the course of evolution, the ancestral...
3.7K
02:54Evolutionary Relationships through Genome Comparisons
6.8K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
6.8K
02:51Multi-species Conserved Sequences
4.6K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
4.6K
01:24Eukaryotic Evolution
40.0K
The endosymbiont theory is the most widely accepted theory of eukaryotic evolution; however, its progression is still somewhat debated. According to the nucleus-first hypothesis, the ancestral prokaryote first evolved a membrane to enclose DNA and form the nucleus. Conversely, the mitochondria-first hypothesis suggests that the nucleus was formed after endosymbiosis of mitochondria.
Contrary to the endosymbiont theory, the eukaryote-first hypothesis proposes that the simpler prokaryotic and...
Contrary to the endosymbiont theory, the eukaryote-first hypothesis proposes that the simpler prokaryotic and...
40.0K
02:37Gene Duplication and Divergence
7.7K
The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
7.7K