Related Experiment Video
Updated: Jan 4, 2026
09:20
Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
Published on: September 2, 2013
28.2K
Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation
Jacek R Wiśniewski1, Katharina Zettl1
1Max Planck Institute of Biochemistry, 82152, Martinsried, Germany.
Data in Brief
|November 1, 2019
Abstract:
Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. Whole cell lysates of Hela cells were processed with FASP using single or double, consecutive or successive, digestion with LysC or trypsin. The generated peptides were analyzed using a LTQ-Orbitrap mass spectrometer. These datasets accompany "Filter Aided Sample Preparation - A Tutorial" (Wiśniewski, 2019).
