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Calcitonin gene-related peptide attenuates angiotensin II-induced ROS-dependent apoptosis in vascular smooth muscle

Hong-Min Luo1, Xia Wu2, Wen-Xuan Liu3

  • 1Department of Nephrology, Third Hospital, Hebei Medical University, Shijiazhuang, China.

Biochemical and Biophysical Research Communications
|November 1, 2019
PubMed

Insights

Calcitonin gene-related peptide (CGRP) protects against angiotensin II-induced apoptosis in vascular smooth muscle cells by reducing reactive oxygen species (ROS) and inhibiting the CaMKII/CREB pathway.

Area of Science:

  • Cardiovascular Biology
  • Cellular Signaling
  • Molecular Medicine

Background:

  • Apoptosis is implicated in cardiovascular diseases.
  • Calcitonin gene-related peptide (CGRP) has protective cardiovascular effects.
  • CGRP's direct impact on angiotensin II-induced vascular smooth muscle cell (VSMC) apoptosis and its mechanism are unknown.

Purpose of the Study:

  • To investigate the effect of CGRP on angiotensin II-induced apoptosis in VSMCs.
  • To elucidate the underlying mechanism of CGRP's anti-apoptotic action.

Main Methods:

  • VSMCs were treated with angiotensin II, CGRP, CGRP receptor antagonist (CGRP8-37), and N-acetyl-L cysteine (NAC).
  • Reactive oxygen species (ROS) production and apoptosis were measured.
  • Activation of CaMKII and CREB signaling pathways was assessed.

Main Results:

  • CGRP significantly suppressed angiotensin II-induced ROS and apoptosis in VSMCs.
  • CGRP receptor antagonism abolished CGRP's protective effects.
  • ROS scavenging with NAC blocked CGRP's effect on apoptosis.
  • CGRP inhibited angiotensin II-induced activation of CaMKII and CREB.

Conclusions:

  • CGRP attenuates angiotensin II-induced apoptosis in VSMCs.
  • This effect is mediated by the reduction of ROS.
  • The mechanism involves the inhibition of the CaMKII/CREB signaling pathway.
Keywords:
Ang IIApoptosisCGRPCREBCaMKIIVSMCs

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