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Updated: Jan 4, 2026

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Novel spa and Multi-Locus Sequence Types (MLST) of Staphylococcus Aureus Samples Isolated from Clinical Specimens in

Yae Sung Mun1, You Jin Hwang2,3,4

  • 1Department of Life science, College of Bio-nano technology, Gachon University, 191, Hambangmoe-ro, Yeonsu-gu, Incheon 21936, Korea. yae01@naver.com.

Antibiotics (Basel, Switzerland)
|November 2, 2019
PubMed

Insights

This study identified a novel Staphylococcus aureus protein A (spa) type, gh22, in multidrug-resistant strains. The findings highlight the emergence of new spa types in methicillin-resistant Staphylococcus aureus infections, posing a global challenge.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genetics

Background:

  • Staphylococcus aureus bacteremia is a frequent and severe global infection.
  • Increasing incidence and diverse Staphylococcus aureus protein A (spa) types present a challenge.
  • Methicillin-resistant Staphylococcus aureus (MRSA) is a significant public health concern.

Purpose of the Study:

  • To investigate a novel spa type of methicillin-resistant Staphylococcus aureus (MRSA).
  • To characterize the antimicrobial resistance patterns and molecular profiles of clinical MRSA isolates.

Main Methods:

  • Antimicrobial susceptibility testing using Kirby-Bauer disk diffusion.
  • Molecular typing via Staphylococcus aureus protein A (spa) and multi-locus sequence typing (MLST) using PCR and sequencing.

Main Results:

  • 55.05% of 109 clinical S. aureus samples were MRSA.
  • A novel spa type, gh22, was detected in five MRSA strains.
  • Identified antibiotic resistance genes (mecA, TEM, aac(6 olyl)-aph(2 olyl)), novel spa types, and undefined MLST.

Conclusions:

  • A high prevalence of spa type t2460 MRSA was observed.
  • Novel spa gh22, MLST ST4613, and CC5 strains exhibiting multidrug resistance were identified.
  • Emergence of novel spa types and multidrug-resistant MRSA strains necessitates ongoing surveillance.
Keywords:
MLSTmethicillin-resistant Staphylococcus aureus (MRSA)multidrug-resistancespaspa typing

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