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Related Concept Videos

Fixation and Sectioning01:03

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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Updated: Jan 4, 2026

Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
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Coloring plastinated specimens.

Stanley Iliff1, Ismael Concha2, Vladimir Chereminskiy3

  • 1DeBusk College of Osteopathic Medicine, Lincoln Memorial University, Harrogate, Tennessee.

Anatomia, Histologia, Embryologia
|November 4, 2019
PubMed
Summary
This summary is machine-generated.

Plastination techniques have evolved significantly, moving beyond basic grey specimens to vibrant, colored anatomical models. Innovations in staining and coloration methods have greatly enhanced the aesthetic and educational value of plastinated biological specimens.

Keywords:
Biodur AC10 stainColored plastinatesRPM stainimidazoleimpregnation mixpaintplastinationsiliconestain

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Area of Science:

  • Anatomical Sciences
  • Biotechnology
  • Medical Education

Background:

  • Early plastination methods produced specimens with a limited, grey/brown color palette, similar to formalin-fixed tissues.
  • Initial attempts to improve coloration using various fixatives were largely unsuccessful.
  • The development of colored epoxy for vascular injections marked a significant advancement in the 1980s.

Observation:

  • The introduction of Biodur AC10® stain was a key step, applied during the final acetone bath for gross specimens.
  • Restoring natural coloration by reactivating red blood cells in formalin-fixed specimens was explored.
  • Direct painting of plastinated vessels showed some success, paving the way for new techniques.

Findings:

  • A proprietary silicone coloration method offered superior results for plastinated specimens.
  • Recent developments include a versatile red pigment stain, further enhancing specimen aesthetics.
  • Various coloration methodologies have been developed and applied to plastination processes.

Implications:

  • Enhanced coloration significantly improves the aesthetic appeal and educational utility of plastinated specimens.
  • Continued innovation in coloration techniques is expected to further advance the field of plastination.
  • The evolution of plastination coloration contributes to more engaging and effective anatomical studies.