Related Experiment Video
Updated: Jan 4, 2026
06:02
A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells
Published on: October 28, 2025
358
Enhancer LncRNAs Influence Chromatin Interactions in Different Ways.
Yue Hou1, Rongxin Zhang1, Xiao Sun1
1State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.
Frontiers in Genetics
|November 5, 2019
Summary
Enhancer RNAs (elncRNAs) reinforce enhancer-promoter interactions, influencing gene regulation. G-quadruplexes on enhancers modulate elncRNA expression and interaction frequency, revealing novel regulatory mechanisms.
Area of Science:
- Genomics
- Molecular Biology
- Epigenetics
Background:
- Over 98% of the human genome is noncoding and understudied.
- Enhancers and non-coding RNAs play crucial roles in gene regulation.
- Enhancer transcripts (elncRNAs) are indicators of enhancer activity and gene regulation.
Purpose of the Study:
- Identify enhancer-promoter interactions using Hi-C data.
- Investigate the role of elncRNAs in enhancer-promoter interactions.
- Explore the influence of G-quadruplexes on these interactions.
Main Methods:
- High-throughput chromosome conformation capture (Hi-C) for interaction mapping.
- Analysis of elncRNA enrichment and expression correlation.
- Identification and analysis of G-quadruplex structures.
Main Results:
- elncRNAs are enriched at chromatin loop anchors and enhance enhancer-promoter interactions.
- elncRNA expression correlates positively with interaction frequency.
- G-quadruplexes mediate specific enhancer-promoter interactions and influence elncRNA expression.
Conclusions:
- elncRNAs play a significant role in reinforcing enhancer-promoter interactions.
- G-quadruplexes are involved in mediating these interactions and regulating elncRNA expression.
- This study provides insights into the regulatory functions of noncoding genomic regions.
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
9.7K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.7K
02:39lncRNA - Long Non-coding RNAs
3.4K
3.4K
02:35Chromatin Position Affects Gene Expression
24.5K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.5K
02:41Chromatin Structure Regulates pre-mRNA Processing
8.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
8.0K
01:32Chromatin Modification in iPS Cells
2.1K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.1K
03:17Inheritance of Chromatin Structures
7.2K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
7.2K