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Updated: Jan 4, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
Published on: March 17, 2010
Kinetic Analysis by Affinity Chromatography
Sazia Iftekhar1, Susan T Ovbude1, David S Hage1
1Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, United States.
Abstract:
Important information on chemical processes in living systems can be obtained by the rates at which these biological interactions occur. This review will discuss several techniques based on traditional and high-performance affinity chromatography that may be used to examine the kinetics of biological reactions. These methods include band-broadening measurements, techniques for peak fitting, split-peak analysis, peak decay studies, and ultrafast affinity extraction. The general principles and theory of each method, as applied to the determination of rate constants, will be discussed. The applications of each approach, along with its advantages and limitations, will also be considered.
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