A sensitive H₂O₂ biosensor based on carbon nanotubes/tetrathiafulvalene and its application in detecting NADH

Reduced nicotinamide adenine dinucleotide (NADH) plays a pivotal role in the electron-transfer chain of biological system. Analysis of many biological markers is based on the detection of the enzymatically generated NADH. In this paper, a sensitive hydrogen peroxide (H₂O₂) biosensor, fabricated by carbon nanotubes (CNTs)/tetrathiafulvalene (TTF)/horseradish peroxidase (HRP), was applied for detecting the NADH in a buffer containing methylene blue (MB) at low operating potential of - 0.3 V (vs. Ag/AgCl). Since the NADH could be oxidized by MB to release H₂O₂, the electrochemical biosensor enables to detect the NADH in the MB buffer. And the low working potential made the biosensor avoid the interference from other electroactive substances. Linear response ranges from 10 μM to 790 μM, with a sensitivity of 4.76 μA mM^-1 and a detection limit of 1.53 μM were obtained under the optimum conditions. The proposed sensor provided a promising approach for sensitively detecting the NADH.