Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Role of SCF/c-KIT axis in pericyte TNT-guided vessel branching.

Fluids and barriers of the CNS·2026
Same author

What is your next step? A case of malabsorption with imitation game.

Internal and emergency medicine·2026
Same author

Endothelial Cells as Antigen-Presenting Cells. An Historical Note.

Clinical anatomy (New York, N.Y.)·2026
Same author

Molecular Mechanisms of Cardiac Fibrosis: A Pathologist's Perspective.

Current issues in molecular biology·2026
Same author

Proteomic profiling of circulating extracellular vesicles from COVID-19 patients and their impact on innate Vdelta2 T-cell response.

Frontiers in immunology·2026
Same author

Tissue characterization in cardiac amyloidosis: a joint consensus document by the gruppo di studio di cardiopatologia (SIAPEC) and the SIC/ANMCO Italian cardiac amyloidosis network (RIAC).

Journal of cardiovascular medicine (Hagerstown, Md.)·2026

Related Experiment Video

Updated: Jan 4, 2026

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
06:32

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Published on: January 9, 2019

8.2K

Dp71 Expression in Human Glioblastoma.

Simona Ruggieri1, Michelina De Giorgis2, Tiziana Annese3

  • 1Department of Basic Medical Sciences, Neurosciences and Sensory Organs, University of Bari Medical School, 70124 Bari, Italy. simona.ruggieri@uniba.it.

International Journal of Molecular Sciences
|November 6, 2019
PubMed
Summary

Reduced Dp71 expression in glioblastoma correlates with increased tumor proliferation and poorer prognosis. This finding highlights Dp71

Keywords:
Dp71glioblastomalamin Btumor progression

More Related Videos

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
09:17

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Published on: September 13, 2022

2.7K
Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
12:52

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

16.4K

Related Experiment Videos

Last Updated: Jan 4, 2026

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
06:32

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Published on: January 9, 2019

8.2K
Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
09:17

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Published on: September 13, 2022

2.7K
Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
12:52

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

16.4K

Area of Science:

  • Neuroscience
  • Oncology
  • Molecular Biology

Background:

  • Dp71, the most abundant dystrophin (DMD) gene product in the nervous system, is linked to cognitive issues in Duchenne muscular dystrophy.
  • The role of Dp71 in tumor progression, particularly in glioblastoma, a primary central nervous system (CNS) tumor, is not well understood.
  • This study focuses on investigating Dp71 expression in glioblastoma.

Purpose of the Study:

  • To investigate the expression levels and localization of Dp71 in glioblastoma.
  • To determine the correlation between Dp71 expression and glioblastoma proliferation.
  • To explore the potential role of Dp71 in glioblastoma prognosis.

Main Methods:

  • Dp71 expression was analyzed using immunofluorescence, immunohistochemistry, RT-PCR, and immunoblotting.
  • Analyses were performed on both glioblastoma cell lines and cells isolated from human glioblastoma multiforme (GBM) specimens.
  • Colocalization studies with lamin B were conducted in normal astrocytes.

Main Results:

  • Dp71 expression was decreased in glioblastoma cell lines and patient samples compared to normal human astrocytes (NHA).
  • Dp71 localized to the nucleus in NHA but shifted to the cytoplasm in glioblastoma cells, colocalizing with lamin B in NHA, suggesting a role in nuclear architecture.
  • Reduced Dp71 protein levels in patient samples showed an inverse correlation with the Ki-67 tumor proliferation index.

Conclusions:

  • Decreased Dp71 expression is significantly associated with increased cancer proliferation in glioblastoma.
  • Lower Dp71 levels correlate with poor prognosis in glioblastoma patients.
  • Dp71 may play a crucial role in regulating glioblastoma cell proliferation and tumor aggressiveness.