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Bipedal DNA walker mediated enzyme-free exponential isothermal signal amplification for rapid detection of microRNA

Anyi Chen1, Ying Zhuo, Yaqin Chai

  • 1Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. yqchai@swu.edu.cn yuanruo@swu.edu.cn.

Chemical Communications (Cambridge, England)
|November 6, 2019
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MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...