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Updated: Jan 4, 2026
Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
Published on: October 4, 2019
Low microRNA-622 expression predicts poor prognosis and is associated with ZEB2 in glioma
Qian Song1, Honggang Pang2, Lei Qi1
1Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, People's Republic of China.
Background:
MicroRNAs have been recently reported to play an important role in tumorigenesis and progression in several forms of tumors. Previous studies have shown that microRNA-622 (miR-622) was associated with glioma proliferation and invasion. However, the clinical significance of miR-622 in glioma has not been elucidated. The aim of our study was to investigate the clinical values of miR-622, as well as investigate the potential molecular mechanisms in glioma.
Materials And Methods:
qRT-PCR and Western blot analysis were used to analyze the expression of miR-622 and ZEB2, respectively. Kaplan-Meier analysis and Cox's proportional hazards model were used in survival analysis. MTT assay, wound healing assay, transwell assay and flow cytometry analysis were carried out to detect the impact of miR-622 on glioma cell proliferation, migration, invasion and apoptosis.
Results:
Our result indicated that miR-622 expression was greatly decreased in glioma tissues and cell lines and the downregulation of miR-622 was significantly associated with the advanced pathological grade and low Karnofsky performance score of glioma. In addition, Kaplan-Meier curves with log-rank analysis revealed a close correlation between downregulation of miR-622 expression and low overall survival rate in glioma patients. Furthermore, Cox regression analysis demonstrated that downregulated miR-622 could be considered as an independent poor prognostic indicator in glioma patients. Finally, our findings demonstrated that miR-622 overexpression remarkably suppressed glioma cell proliferation, migration and invasion, while facilitated apoptosis by suppressing ZEB2 in vitro.
Conclusion:
Our study suggested that miR-622 may be identified as a valuable prognostic biomarker and a promising therapeutic target for glioma patients.
Insights
MicroRNA-622 (miR-622) is downregulated in glioma, correlating with poor prognosis. Restoring miR-622 suppresses tumor growth and invasion, indicating its potential as a biomarker and therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- MicroRNAs are implicated in cancer development and progression.
- MicroRNA-622 (miR-622) has been linked to glioma cell proliferation and invasion.
- The clinical significance of miR-622 in glioma remains unclear.
Purpose of the Study:
- To investigate the clinical value of miR-622 in glioma.
- To explore the molecular mechanisms underlying miR-622's role in glioma.
Main Methods:
- Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot for miR-622 and ZEB2 expression.
- Kaplan-Meier analysis and Cox regression for survival analysis.
- In vitro assays (MTT, wound healing, Transwell, flow cytometry) to assess miR-622's functional impact on glioma cells.
Main Results:
- miR-622 expression was significantly decreased in glioma tissues and cell lines.
- Downregulation of miR-622 correlated with advanced pathological grade and poor Karnofsky performance score.
- Low miR-622 expression predicted a poor overall survival rate and served as an independent prognostic indicator.
- miR-622 overexpression inhibited glioma cell proliferation, migration, and invasion, while promoting apoptosis by suppressing ZEB2.
Conclusions:
- miR-622 may serve as a valuable prognostic biomarker for glioma patients.
- miR-622 represents a promising therapeutic target for glioma treatment.
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