Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

718
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
718
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.9K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.9K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

14.6K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
14.6K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.2K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.2K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

983
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
983

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The role of sex in structuring aggression, rank, and hierarchy in monk parakeets.

Integrative and comparative biology·2026
Same author

Effect of solvents on multifunctional Dy<sub>2</sub> complexes with axial chiral ligands (<i>R</i>)/(<i>S</i>)-1,1'-binaphthyl-2,2'-diyl phosphate.

Dalton transactions (Cambridge, England : 2003)·2026
Same author

Fibroblast-Mimetic Lignin Polymersomes for Logic-Gated Synthesis of Mechanically Reconfigurable Bioskins.

Angewandte Chemie (International ed. in English)·2026
Same author

Integrative profiling of glymphatic dysfunction in adolescent subthreshold depression.

Journal of affective disorders·2026
Same author

Recent advances in nanomaterial-based strategies for chronic pain alleviation.

Materials today. Bio·2026
Same author

ProtFormer-Site: Ultra-fast and Accurate Prediction of Protein-Protein Interaction Sites with Protein Language Model.

Interdisciplinary sciences, computational life sciences·2026
Same journal

In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

Journal of microscopy·2026
Same journal

The future of DXA: How AI is transforming bone health diagnostics.

Journal of microscopy·2026
Same journal

The Origins of Ploem's Filter Cube: A Pandora's Box.

Journal of microscopy·2026
Same journal

The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

Journal of microscopy·2026
Same journal

Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

Journal of microscopy·2026
Same journal

Modular training resources for bioimage analysis.

Journal of microscopy·2026
See all related articles

Related Experiment Video

Updated: Jan 4, 2026

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy
06:37

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

Published on: June 15, 2022

4.1K

Image scanning difference microscopy.

Yuchen Chen1, Shaocong Liu1, Chengfeng Zhang1

  • 1State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China.

Journal of Microscopy
|November 7, 2019
PubMed
Summary
This summary is machine-generated.

We developed image scanning difference microscopy (ISDM), a novel superresolution imaging technique. ISDM enhances fluorescence emission difference (FED) microscopy, achieving 111 nm resolution with reduced artifacts for versatile biological studies.

Keywords:
Fluorescence microscopyparallel detectionsuperresolution

More Related Videos

Phase Contrast and Differential Interference Contrast DIC Microscopy
06:49

Phase Contrast and Differential Interference Contrast DIC Microscopy

Published on: August 6, 2008

53.6K
Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.6K

Related Experiment Videos

Last Updated: Jan 4, 2026

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy
06:37

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

Published on: June 15, 2022

4.1K
Phase Contrast and Differential Interference Contrast DIC Microscopy
06:49

Phase Contrast and Differential Interference Contrast DIC Microscopy

Published on: August 6, 2008

53.6K
Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.6K

Area of Science:

  • Optical microscopy
  • Superresolution imaging
  • Biophysics

Background:

  • Fluorescence emission difference (FED) microscopy offers a simple, versatile superresolution method.
  • FED microscopy faces challenges with negative value distortions, limiting further development.
  • Existing superresolution techniques like STED require complex setups and specific dyes.

Purpose of the Study:

  • To introduce a novel superresolution imaging method, image scanning difference microscopy (ISDM).
  • To overcome limitations of FED microscopy, specifically negative value artifacts.
  • To enhance resolution and image quality in superresolution microscopy.

Main Methods:

  • Implementation of ISDM utilizing a detector array of 19 avalanche photodiodes (APD).
  • Integration of ISDM with the established fluorescence emission difference (FED) method.
  • Parallel detection system for improved signal-to-noise ratio (SNR) and artifact reduction.

Main Results:

  • Achieved a lateral resolution of 111 nm (approximately λ/6), the highest reported for FED.
  • Significantly reduced artifacts caused by negative values inherent in FED.
  • Demonstrated a simple, versatile setup with no complex alignment or dye constraints.

Conclusions:

  • ISDM represents a significant advancement in superresolution imaging, building upon FED principles.
  • The method offers high resolution, improved image quality, and ease of use.
  • ISDM is a promising tool for biological and fundamental scientific research.