Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

3.4K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
3.4K
Cell Migration01:09

Cell Migration

18.4K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.4K
Cell Migration01:19

Cell Migration

6.3K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.3K
Role of Myosin in Cell Migration01:18

Role of Myosin in Cell Migration

3.1K
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II  is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
3.1K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

5.4K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.4K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

2.4K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CBCT-MRI-based prediction models for stratifying anterior disc displacement in orthodontic patients: development and independent internal validation of a retrospective diagnostic study.

Oral radiology·2026
Same author

A functionally guided fusion Vision Transformer for predicting IDH status in gliomas: a multicenter study with external validation and incomplete multimodal evaluation.

Radiologie (Heidelberg, Germany)·2026
Same author

Association of Lumbar Sagittal Curvature Profiles with Musculoskeletal Disorders: A Pilot Radiographic Study.

Diagnostics (Basel, Switzerland)·2026
Same author

(-)-Guaiol inhibits lung cancer via PPARG-dependent fatty acid oxidation.

Neoplasma·2026
Same author

The Osteoblastic Microenvironment Determines the Fate of Breast Cancer Cells Disseminated in the Bone Marrow.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

(-)-Guaiol Downregulates M2 Tumor-Associated Macrophage Polarization Through PPAR-γ Signaling to Suppress Lung Cancer.

Biological procedures online·2025

Related Experiment Video

Updated: Jan 4, 2026

Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events
08:30

Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events

Published on: August 27, 2019

8.4K

Migration regulates cellular mechanical states.

Stephanie S Chang1, Andrew D Rape1, Stephanie A Wong1

  • 1Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213.

Molecular Biology of the Cell
|November 7, 2019
PubMed
Summary
This summary is machine-generated.

Cell migration state regulates cell traction forces by altering focal adhesion dynamics. Stationary cells exhibit stronger, less dynamic forces, impacting cell growth and differentiation.

More Related Videos

A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro
09:50

A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro

Published on: August 27, 2015

8.6K
Integrative Toolkit to Analyze Cellular Signals: Forces, Motion, Morphology, and Fluorescence
14:55

Integrative Toolkit to Analyze Cellular Signals: Forces, Motion, Morphology, and Fluorescence

Published on: March 5, 2022

4.3K

Related Experiment Videos

Last Updated: Jan 4, 2026

Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events
08:30

Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events

Published on: August 27, 2019

8.4K
A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro
09:50

A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro

Published on: August 27, 2015

8.6K
Integrative Toolkit to Analyze Cellular Signals: Forces, Motion, Morphology, and Fluorescence
14:55

Integrative Toolkit to Analyze Cellular Signals: Forces, Motion, Morphology, and Fluorescence

Published on: March 5, 2022

4.3K

Area of Science:

  • Cell Biology
  • Biophysics
  • Mechanobiology

Background:

  • Adherent cells sense and respond to their physical environment.
  • Mechanical forces are crucial for cell sensing, regulating growth and differentiation.
  • The role of cell migration state in force generation is not well understood.

Purpose of the Study:

  • To investigate how cell migration state influences cellular traction forces.
  • To elucidate the relationship between cell migration, focal adhesion dynamics, and force generation.

Main Methods:

  • Utilized traction force microscopy to measure forces generated by migrating and stationary cells.
  • Analyzed focal adhesion turnover and paxillin phosphorylation.
  • Employed checkerboard micropatterns to guide cell migration and observe focal adhesion formation/down-regulation.

Main Results:

  • Stationary cells generate stronger, less dynamic, and more peripheral traction forces than migrating cells.
  • Reduced focal adhesion turnover and increased paxillin phosphorylation correlate with stationary states.
  • New focal adhesion formation in migrating cells is linked to the down-regulation of existing adhesions and forces.

Conclusions:

  • Cell migration dynamically regulates traction forces through focal adhesion turnover.
  • This mechanism links cell migration state to cellular processes like wound healing and embryogenesis.
  • Coordination of cell differentiation with migration state and localization is critical.