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A transparent paper-based platform for multiplexed bioassays by wavelength-dependent absorbance/transmittance
Lijun Zong1, Yufeng Han, Lei Gao
1Institute of Advanced Materials (IAM) & Key Laboratory of Flexible Electronics (KLoFE), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing 211816, PR China. iamjhliu@njtech.edu.cn.
The Analyst
|November 8, 2019
Summary
Researchers developed a transparent paper-based sensor for detecting multiple targets like bovine serum albumin (BSA) and cholesterol. This cost-effective and sensitive platform shows promise for advanced multiplexed bioassays.
Area of Science:
- Analytical Chemistry
- Biomedical Engineering
- Materials Science
Background:
- Multiplexed bioassays are crucial for simultaneous detection of multiple analytes in biological samples.
- Existing methods often require complex instrumentation and high costs, limiting their accessibility.
- Paper-based sensing platforms offer a low-cost, portable, and disposable alternative for point-of-care diagnostics.
Purpose of the Study:
- To design and develop a novel transparent paper-based chemosensing platform.
- To enable multi-target detection using wavelength-dependent absorbance/transmittance.
- To demonstrate the platform's efficacy in analyzing real biological samples like serum.
Main Methods:
- Rational design of a transparent paper substrate incorporating specific chemosensors.
- Utilizing wavelength-dependent absorbance and transmittance for signal transduction.
- Application of the platform for the detection of bovine serum albumin (BSA) and cholesterol in serum samples.
Main Results:
- Successful development of a transparent paper-based chemosensing platform.
- Achieved low detection limits for bovine serum albumin (BSA) at 0.1 μM and cholesterol at 0.1 mM.
- Demonstrated effective multi-target detection capabilities in serum samples.
Conclusions:
- The developed transparent paper-based platform is a cost-effective and highly sensitive tool for multiplexed bioassays.
- The platform's ability to detect multiple targets with high sensitivity shows significant potential for clinical diagnostics and research.
- This technology paves the way for accessible and advanced point-of-care testing solutions.
