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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
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A beginner's guide for FMDV quasispecies analysis: sub-consensus variant detection and haplotype reconstruction using
Marco Cacciabue1,2, Anabella Currá1,2, Elisa Carrillo1
1Instituto de Agrobiotecnología y Biología Molecular (IABiMo, INTA-CONICET), Hurlingham, Argentina.
Briefings in Bioinformatics
|November 8, 2019
Summary
This guide simplifies analyzing viral quasispecies from Illumina sequencing data, making RNA virus complexity accessible. It provides a pipeline for exploring sub-consensus variability and reconstructing viral populations.
Area of Science:
- Virology
- Bioinformatics
- Genomics
Background:
- Deep sequencing of viral genomes is crucial for understanding RNA virus complexity.
- Analyzing next-generation sequencing data for viral quasispecies can be challenging for novice researchers.
- Viral quasispecies represent a dynamic population of closely related viral genomes.
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