Synthesis, radiosynthesis, and positron emission tomography neuroimaging using 5-[¹⁸ F]fluoro-L-amino suberate

System xc- (Sx_c -) has emerged as a new biological target for PET studies to detect oxidative and excitotoxic stress. Notably, applications have, thus far, been limited to tumour imaging although Sx_c^- ) may play a major role in neurodegeneration. The synthesis procedures of tosylate precursor and its translation to Sx_c - PET tracer 5[18F]fluoro-L-amino suberate by manual and automated radiosyntheses are described. A brain-PET study has been conducted to evaluate the tracer uptake into brain in healthy mice.