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Midgut transcriptome analysis of Clostera anachoreta treated with lethal and sublethal Cry1Ac protoxin
Jie Liu1, Liucheng Wang1, Guona Zhou1
1Ecological Laboratory, Forestry College, Agricultural University of Hebei, Baoding, China.
Archives of Insect Biochemistry and Physiology
|November 9, 2019
Summary
This study identified six genes and the PI3K-Akt pathway in the poplarimporting moth (Clostera anachoreta) that may be involved in its response to Cry1Ac toxin. These findings could aid in developing biological control methods for this forestry pest.
Area of Science:
- Entomology
- Molecular Biology
- Toxicology
Background:
- Clostera anachoreta is a significant Lepidoptera pest affecting poplar plantations globally.
- This insect is a known target of Cry1Ac toxin produced by Bacillus thuringiensis (Bt) plants.
Purpose of the Study:
- To investigate the molecular mechanisms underlying the response of Clostera anachoreta to Cry1Ac toxin stress.
- To identify key genes and pathways involved in Cry1Ac toxin resistance and midgut repair.
Main Methods:
- Transcriptome analysis of Clostera anachoreta exposed to Cry1Ac toxin.
- Bioinformatic analysis to identify differentially expressed genes and enriched pathways.
Main Results:
- Six genes (HSC70, GNB2L/RACK1, PNLIP, BI1-like, arylphorin type 2, and PKM) were identified as potentially associated with Cry1Ac toxin response.
- The PI3K-Akt signaling pathway was significantly enriched and linked to other crucial pathways (B-cell receptor, toll-like receptor, MAPK).
- These pathways are suggested to play a role in the midgut recovery following sublethal Cry1Ac toxin exposure.
Conclusions:
- This is the first large-scale sequencing study on C. anachoreta's response to Cry toxin stress.
- Identified genes and pathways offer potential targets for managing Bt-cry1Ac resistance and developing novel biological control strategies against this pest.
