Midgut transcriptome analysis of Clostera anachoreta treated with lethal and sublethal Cry1Ac protoxin

Clostera anachoreta is one of the important Lepidoptera insect pests in forestry, especially in poplars woods in China, Europe, Japan, and India, and so forth, and also the target insect of Cry1Ac toxin and Bt plants. Six genes, HSC70, GNB2L/RACK1, PNLIP, BI1-like, arylphorin type 2, and PKM were found in this study, and they might be associated with the response to the Cry1Ac toxin, found by analyzing the transcriptome data. And the PI3K-Akt pathway was highly enriched in differentially expressed unigenes and linked to several crucial pathways, including the B-cell receptor signaling pathway, toll-like receptor pathway, and mitogen-activated protein kinase signaling pathway. They might be involved in the recovery stage of the damaged midgut during the response to sublethal doses of Cry1Ac toxin. This is the first study conducted to specifically investigate C. anachoreta response to Cry toxin stress using large-scale sequencing technologies, and the results highlighted some important genes and pathways that could be involved in Btcry1Ac resistance development or could serve as targets for biologically based control mechanisms of this insect pest.