Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1
Meng-Hsuan Wen1, Xihong Xie1, Jian Tu2
1Department of Chemistry, University of Houston, Houston, Texas 77204, United States.
Abstract:
ATOX1 is a copper chaperone involved in intracellular copper homeostasis, cell proliferation, and tumor progression. To investigate the physiologically relevant molecular mechanism of ATOX1 by using imaging-based approaches, we genetically modified ATOX1 in H1 hESCs to express mCherry-ATOX1 fusion protein under endogenous regulatory machinery. The fluorescence engineered hESC clone maintains characteristic stem cell features and can differentiate to all three germ layers, serving as a unique tool to dissect the role of ATOX1 in various cellular processes.
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