Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

1.4K
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
1.4K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.4K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.4K
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

1.2K
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
1.2K
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

1.2K
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
1.2K
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

1.0K
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
1.0K
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

1.6K
The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A mathematical model of NMR transverse relaxation for pore size distribution estimation in porous media.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2025
Same author

NMR Relaxation by Redfield equation in a spin system I=7/2.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2023
Same author

Saturation-recovery as a T<sub>1</sub>-filter for T<sub>2</sub>-T<sub>2</sub> exchange NMR.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2019
Same author

Spin squeezing in a quadrupolar nuclei NMR system.

Physical review letters·2015
Same author

Measuring bipartite quantum correlations of an unknown state.

Physical review letters·2014
Same author

Environment-induced sudden transition in quantum discord dynamics.

Physical review letters·2011
Same journal

Localization-driven exchange contrast in diffusion exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

4.5 Tesla superconducting miniature magnet in liquid nitrogen.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Folding and unfolding dynamics of a DNA aptamer studied by heteronuclear <sup>1</sup>H-<sup>13</sup>C correlation zz-exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Multi-spin control from one-spin pulses.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Altering MRI rotating frame relaxations by changing the truncation level of Hyperbolic Secant pulse.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Effects of proton exchange on the lifetimes of long-lived states in aliphatic chains.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
See all related articles

Related Experiment Video

Updated: Jan 4, 2026

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.6K

Direct NMR T1 distribution measurement without using ill-posed fitting methods.

E T Montrazi1, T J Bonagamba1

  • 1São Carlos Institute of Physics, University of São Paulo, PO Box 369, 13560-970 São Carlos, SP, Brazil.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|November 10, 2019
PubMed
Summary
This summary is machine-generated.

This study introduces a novel Saturation-Inversion-Recovery (SIR) method for Nuclear Magnetic Resonance (NMR) to directly measure spin-lattice relaxation time (T1) distributions, bypassing complex curve fitting. This approach enhances accuracy in T1 measurements for various scientific applications.

Keywords:
Combined saturation-inversion-recoveryDSIR-T(1)Direct T(1) distribution measurement by SIRNMRSIRSpin-lattice relaxationT(1) distribution

More Related Videos

A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy
09:32

A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy

Published on: January 30, 2019

15.0K
15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

5.9K

Related Experiment Videos

Last Updated: Jan 4, 2026

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.6K
A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy
09:32

A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy

Published on: January 30, 2019

15.0K
15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

5.9K

Area of Science:

  • Magnetic Resonance Spectroscopy
  • Biophysical Chemistry
  • Materials Science

Background:

  • Traditional spin-lattice relaxation time (T1) distribution measurements using Inversion-Recovery (IR) and Saturation-Recovery (SR) rely on ill-posed multi-exponential curve fitting.
  • A recently developed Saturation-Inversion-Recovery (SIR) method offers improved T1-dependence sharpness compared to IR and SR.

Purpose of the Study:

  • To propose an optimized combination of SIR signals for direct T1 distribution acquisition.
  • To eliminate the need for multi-exponential fitting in T1 measurements.

Main Methods:

  • Utilizing the Saturation-Inversion-Recovery (SIR) pulse sequence.
  • Developing a novel signal processing strategy for direct T1 distribution calculation from SIR data.

Main Results:

  • Demonstration of a method to directly obtain T1 distributions using combined SIR signals.
  • Elimination of reliance on traditional, potentially inaccurate, multi-exponential fitting algorithms.

Conclusions:

  • The proposed SIR signal combination provides a more direct and potentially more accurate method for T1 distribution analysis.
  • This advancement simplifies T1 measurement, improving its applicability in diverse scientific fields.