Gold nanoparticles-based biosensor can detect drought stress in tomato by ultrasensitive and specific determination...

Drought stress can significantly affect the yield and quality of tomato production. However, the development of a sensitive and specific method for the determination of drought stress is somehow challenging since plant common morpho-physiological and biochemical characteristic are not generally specific to biotic and abiotic stresses. As a solution, the concentration of miRNAs in plant tissues can be a selective and specific indicator of plant stress. In this study, an optical biosensor based on gold nanoparticles is introduced to determine miRNA-1886 in tomato plant roots. Results showed that irrigation levels from 100% to 60% of field capacity increased the concentration of miRNA-1886 in a range from ca. 100 to 6800 fM (fM) causing a linear change in the biosensor response (R² = 0.97). Results also revealed that in contrast with plant conventional morpho-physiological and biochemical characteristic, miRNA-1886 concentration was not significantly affected (P < 0.01) by other stresses, i.e., salinity and temperature during the growth period. The biosensor introduced in this study is a reliable method to study stress-related functions of miRNAs in plants and their application in specific plant stress determination.