Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transmission Electron Microscopy01:15

Transmission Electron Microscopy

6.7K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
6.7K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

12.8K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
12.8K
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

590
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
590
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

1.4K
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
1.4K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.8K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization and modulation of human insulin degrading enzyme conformational dynamics to control enzyme activity.

eLife·2026
Same author

A bacteriophytochrome Pr/Pfr heterodimer studied through single-particle time-resolved cryo-electron microscopy.

Communications chemistry·2026
Same author

Critical amino acid residues in the N-terminal domain of NADPH-dependent assimilatory sulfite reductase flavoprotein mediate octameric assembly.

bioRxiv : the preprint server for biology·2026
Same author

Time-Resolved Fluorescence Imaging and Correlative Cryo-Electron Tomography to Study Structural Changes of the HIV-1 Capsid.

ACS nano·2025
Same author

Structure of dimerized assimilatory NADPH-dependent sulfite reductase reveals the minimal interface for diflavin reductase binding.

Nature communications·2025
Same author

Multiple roles for TFG ring complexes in neuronal cargo trafficking.

bioRxiv : the preprint server for biology·2024
Same journal

Towards light-coupled sample preparation for time-resolved cryoEM studies.

IUCrJ·2026
Same journal

Cryo-EM analysis of cooperative conformational changes in the SARS-CoV-2 spike protein trimer.

IUCrJ·2026
Same journal

Towards time-resolved MicroED grid preparation using mix-and-inject gas dynamic virtual nozzles.

IUCrJ·2026
Same journal

How cryoEM has advanced our understanding of bacteriophages and bacteriocins targeting Clostridioides difficile.

IUCrJ·2026
Same journal

CryoEM structures reveal allosteric regulation of the catalytic activity of the multi-protein human MAT enzyme complexes.

IUCrJ·2026
Same journal

Cryo-EM-guided subtractive optimization of a novel VCP/p97 inhibitor.

IUCrJ·2026
See all related articles

Related Experiment Video

Updated: Jan 4, 2026

Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

10.1K

Throughput and resolution with a next-generation direct electron detector.

Joshua H Mendez1, Atousa Mehrani2, Peter Randolph3

  • 1Department of Physics, Florida State University, 77 Chieftan Way, Tallahassee, FL 32306, USA.

Iucrj
|November 12, 2019
PubMed
Summary
This summary is machine-generated.

The new DE64 direct electron detector (DED) excels in counting mode for cryo-electron microscopy (cryo-EM), achieving high resolution. Superior image quality in counting mode is crucial for cryo-EM reconstructions, outweighing faster data collection in integrating mode.

Keywords:
3D reconstructionadvances in microscope hardwaredirect electron detectorsimage processingsingle-particle cryo-EM

More Related Videos

All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics
11:33

All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics

Published on: January 19, 2018

10.2K
Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2
11:27

Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2

Published on: December 8, 2016

12.7K

Related Experiment Videos

Last Updated: Jan 4, 2026

Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

10.1K
All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics
11:33

All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics

Published on: January 19, 2018

10.2K
Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2
11:27

Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2

Published on: December 8, 2016

12.7K

Area of Science:

  • Structural Biology
  • Microscopy Technology
  • Biophysics

Background:

  • Direct electron detectors (DEDs) have significantly advanced cryo-electron microscopy (cryo-EM) by enabling motion correction and high-resolution imaging.
  • The DE64, a next-generation DED, offers dual functionality in both integrating and counting modes.

Purpose of the Study:

  • To characterize the performance of the DE64 DED in both integrating and counting modes for cryo-EM applications.
  • To compare the impact of different detection modes and imaging conditions on cryo-EM reconstruction resolution and data collection efficiency.

Main Methods:

  • Characterization of the DE64 DED using modulation transfer function, noise power spectrum, and detective quantum efficiency (DQE) measurements.
  • Comparison of data collection throughput and image quality between integrating and counting modes.
  • Cryo-EM data collection and reconstruction of apoferritin using integrating mode, integrating mode with a Volta phase plate (VPP), and counting mode.

Main Results:

  • The DE64 exhibited superior detective quantum efficiency (DQE) in counting mode compared to integrating mode.
  • Data collection throughput was over ten times slower in counting mode than in integrating mode.
  • Only counting mode data enabled a better than 3 Å resolution reconstruction for apoferritin, even with fewer particles, demonstrating the importance of image quality.

Conclusions:

  • Superior image quality in counting mode is more critical for achieving high-resolution cryo-EM reconstructions than the higher throughput of integrating mode.
  • The DE64's counting mode performance is essential for advancing high-resolution cryo-EM, surpassing the benefits of increased throughput or contrast enhancement in integrating mode.