Related Experiment Video
Updated: Jan 4, 2026
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method
Published on: October 7, 2025
Highly specific and sensitive detection of microRNAs by tandem signal amplification based on duplex-specific nuclease
Qing Liu1, Peng-Jian Kang1, Zeng-Ping Chen1
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. zpchen@hnu.edu.cn.
Abstract:
Based on the full understanding of the hydrolysis patterns of duplex-specific nuclease (DSN) against the probe DNAs in DNA/microRNA heteroduplexes, a simple and generic platform for highly specific and sensitive detection of microRNAs was developed by seamlessly integrating DSN-assisted target recycling amplification and strand displacement amplification in tandem.
