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Updated: Jan 4, 2026

Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay
Published on: February 9, 2021
Spiroalanfurantones A-D, Four Eudesmanolide-Furan Sesquiterpene Adducts with a Pentacyclic 6/6/5/5/5 Skeleton from
You-Sheng Cai1, Zi Wu1, Jian-Rong Wang2
1Institute of TCM and Natural Products, School of Pharmaceutical Sciences , Wuhan University , Wuhan 430071 , People's Republic of China.
Abstract:
Spiroalanfurantones A-D (1-4), four eudesmanolide-furan sesquiterpene adducts with an unprecedented pentacyclic 6/6/5/5/5 skeleton, were isolated from the roots of Inula helenium. Their structures were elucidated by spectroscopic data analysis and single-crystal X-ray diffraction analysis. The plausible biosynthetic pathway for 1-4 is presented. Bioassay showed that compounds 1 and 2 significantly inhibited nitric oxide production in lipopolysaccharide-induced RAW264.7 macrophages with IC50 values of 17.3 and 9.5 μM, respectively.
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