Flavicella sediminum sp. nov., isolated from marine sediment

A novel, Gram-stain-negative, motile, flagellated, aerobic, rod-shaped (0.5-0.7 µm wide and 1.0-1.2 µm long) and faint-yellow strain, designated ALS 84^T, was isolated from marine sediment sampled at Ailian bay, Rongcheng, PR China. Growth occurred in the presence of 1-3 % (w/v) NaCl (optimum, 2 % NaCl), at pH 4.0-8.0 (pH 6.0-7.0) and at 8-30 °C (28 °C). The genome size was 4.37 Mbp. The G+C content of the genomic DNA was 33.6 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences suggested that strain ALS 84^T belongs to the genus Flavicella within the family Flavobacteriaceae, and is most closely related to Flavicella marina (95.6 % similarity). The major fatty acids (>10 %) were iso-C_{15 : 0} 3-OH (22.9 %), iso-C_{15 : 0} (14.0 %) and C_{16 : 0} (10.9 %). The major polar lipids were phosphatidylethanolamine and three unidentified lipids. Menaquinone-6 (MK-6) was identified as the respiratory quinone. On the basis of the phenotypic, phylogenetic and chemotaxonomic data obtained in the study, strain ALS 84^T is considered to represent a novel species of the genus Flavicella, for which the name Flavicella sediminum sp. nov. is proposed. The type strain of the novel species is strain ALS 84^T (=KCTC 62398^T=MCCC 1K03480^T).